| No Strep, 10-6 dilution
| |||
|
| Plate 1 (colonies)
| Plate 2 (colonies)
| Average (colonies)
|
| Dalia
| 212
| 140
| 176
|
| Kendra
| 200
| 113
| 157
|
| Muhammad
| 200
| 133
| 169
|
| Haripreet/Mike
| 143
| 177
| 160
|
| Average
| 190
| 141
| 166
|
|
| # of Mutant Colonies
|
|
Dalia
|
14
|
|
Kendra
|
6
|
|
Muhammad
|
3
|
|
Haripreet/Mike
|
1
|
|
Average
|
6
|
| Re-patched Strep Plates
| |||
|
| StrR
| StrD
| StrI
|
|
Dalia
|
14
|
0
|
<1
|
|
Kendra
|
3
|
3
|
<1
|
|
Muhammad
|
3
|
0
|
<1
|
|
Haripreet/Mike
|
1
|
0
|
<1
|
|
Average
|
5
|
1
|
<1
|
Viable Cell Count= Dilution Factor x Final Concetration
1.66 X 109 cfu/mL =(106 dilution)(166 colonies/0.1 mL) (cfu= colony forming unit)
(mfu= mutant forming unit)
StrR Mutant Colonies: 5 mfu/ mL
StrR Muation Frequency: (5mfu/mL )/( 1.66 X 109 cfu/mL)= 3.0 X 10-9 mutants/viable cells
StrD Mutant Colonies: 1mfu/mL
StrD Muation Frequency: (1mfu/mL )/( 1.66 X 109 cfu/mL)= 6.0 X 10-10 mutants/viable cells
StrI Mutant Colonies: <1mfu/mL
StrI Muation Frequency: (<1mfu/mL )/( 1.66 X 109 cfu/mL)= <6.0 X 10-10 mutants/viable cells
